Shinwa Chemical Industries Ltd.
LC/GC Search / Application Note

Shinwa Chemical Industries
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"Uric acid" Filtered 15 Item.

Graph

GC

Samples
Caprylic Acid , Capric Acid , Lauric Acid , Myristic Acid , Palmitic Acid , Stearic Acid , Oleoic Acid
Applications
Free fatty acid in the cocoanut oil
Column
Advance-DS 5% Shinchrom A 80〜100(DMCS)
Column Size
2.1m×3.2mm
Column temp.
225°C
Carrier gas
N2,60ml/min
Injection port temp.
260°C
Detector temp.
260°C(FID)

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GC

Samples
Capric Acid Methyl Ester , Lauric Acid Methyl Ester , Myristic Acid Methyl Ester , Palmitic Acid Methyl Ester , Palmitoleic Acid Methyl Ester , Stearic Acid Methyl Ester , Oleoc Acid Methyl Ester , Linoleic Acid Methyl Ester , Linoelaidic Acid Methyl Ester , Arachidic Acid Methyl Ester , cis-11-Eicosenoic Acid Methyl Ester , Behenic Acid Methyl Ester , Erucic Acid Methyl Ester , 7,10,13,16-Docatetranoic Acid Methyl Ester , Lignoceric Acid Methyl Ester , Nervonic Acid Methyl Ester
Applications
Fatty acid methyl esters
Column
ULBON HR-20M
Column Size
25m×0.25mm(0.25μm)
Column temp.
130°C→240°C(4°C/min)
Carrier gas
He,1.1ml/min
Detector temp.
250°C(FID)
Injection volume
1.0μm

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GC

Samples
Capric Acid Methyl Ester , Lauric Acid Methyl Ester , Myristic Acid Methyl Ester , Palmitic Acid Methyl Ester , Oleoic Acid Methyl Ester , Stearic Acid Methyl Ester , Elaidic Acid Methyl Ester , Linoelaidic Acid Methyl Ester , Linoleic Acid Methyl Ester , Linolenic Acid Methyl Ester , Arachidic Acid Methyl Ester , cis-11-Eicosenoic Acid Methyl Ester , Behenic Acid Methyl Ester , Erucic Acid Methyl Ester , Lignoceric Acid Methyl Ester , Nervonic Acid Methyl Ester , 4,7,10,13,16,19-Docosahexaenoic Acid Methyl Ester
Applications
Standard data Fatty acid methyl ester standard (2)
Column
ULBON HR-SS-10
Column Size
50m×0.32mm
Column temp.
150°C→220°C(3°C/min)
Carrier gas
He,3.4ml/min
Detector temp.
250°C(FID)

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Graph

GC

Samples
Capric Acid Methyl Ester , Lauric Acid Methyl Ester , Myristic Acid Methyl Ester , Palmitic Acid Methyl Ester , Palmitoleic Acid Methyl Ester , Stearic Acid Methyl Ester , Oleoic Acid Methyl Ester , Linoleic Acid Methyl Ester , Linolenic Acid Methyl Ester , Arachidic Acid Methyl Ester , cis-11-Eicosenoic Acid Methyl Ester , Behenic Acid Methyl Ester , Erucic Acid Methyl Ester , 4,7,10,13,16,19-Docosahexaenoic Acid Methyl Ester , Lignoceric Acid Methyl Ester , Nervonic Acid Methyl Ester
Applications
Standard data Fatty acid methyl ester standard (1)
Column
ULBON HR-Thermon-3000B
Column Size
50m×0.25mm
Column temp.
180°C→220°C(1°C/min)
Carrier gas
He,1.7ml/min
Detector temp.
250°C(FID)

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GC

Samples
long-chain fatty acid methyl esters (FAMEs)
Applications
Measurement of long-chain fatty acid methyl esters (FAMEs C10~C24) using Shinwasorb
Column
Shinchrom E71 5% Shinwasorb-U 60/80
Column Size
3.1 m x 3.2 mm I.D. Glass
Column temp.
230℃
Carrier gas
40 mL/min N₂
Injection port temp.
250℃
Detector temp.
250℃
Detector
FID
Injection volume
1.0 μL

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GC

Samples
palm oil
Applications
Measurement of fatty acid methyl esters (palm oil) using Shinwasorb
Column
Shinchrom E71 5% Shinwasorb-U 60/80
Column Size
3.1 m x 3.2 mm I.D. Glass
Column temp.
210℃
Carrier gas
40 mL/min N₂
Injection port temp.
260℃
Detector temp.
260℃
Detector
FID
Injection volume
1.0 μL

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LC

Samples
Hippuric acid , o-Methyl hippuric acid , p-Methyl hippuric acid , m-Methyl hippuric acid
Applications
Hippuric acid
Column
STR ODS-II
Column Size
150 mm L × 4.6 mm I.D.
Mobile phase
20 mM Phosphoric acid buffer (pH 2.5) + 10 mM β-Cyclodextrin/CH3CN = 8/1
Frow rate
1.0 mL/min
Column temp.
50°C

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LC

Samples
Uric acid, Hypoxanthine, Xanthine, Inosine
Applications
Uric acid, Hypoxanthine, Xanthine, Inosine
Column
ULTRON VX-ODS
Column Size
150 mm L × 4.6 mm I.D.
Mobile phase
20 mM Phosphoric acid buffer(pH 6.7)/CH3OH = 99/1
Frow rate
1.0 mL/min
Column temp.
25°C

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LC

Samples
Allantoin , Xanthine , Hypoxanthine , Uric acid
Applications
Uric acid metabolites
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
10 mM CH3CO2NH4(pH 4.7)/CH3CN = 10/90
Frow rate
0.35 mL/min
Column temp.
30°C
Injection volume
1.0 µL

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LC

Samples
Urea , Xanthine , Lactic acid , Creatinine , Uric acid
Applications
Sweat Composition
Column
ULTRON AF-HILIC-CD (PLSS)
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
10 mM HCO2NH4(pH 3.0)/CH3CN = 10/90
Frow rate
0.30 mL/min
Column temp.
30°C
Injection volume
1.0 µL

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LC

Samples
Caffeine , Theobromine , Purine , Xanthine , Hypoxanthine , Adenine , Guanine , Uric acid , Isoguanine
Applications
Purine and purine bases
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
15 mM CH3CO2NH4(pH 4.7)/CH3CN = 10/90
Frow rate
0.35 mL/min
Column temp.
30°C
Injection volume
1.0 µL

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LC

Samples
Cyanuric acid , Ammelide , Melamine , Ammeline
Applications
Melamine-related compounds
Column
ULTRON AF-HILIC-CD
Column Size
100 mm L × 2.0 mm I.D.
Mobile phase
20 mM HCO2NH4(pH 6.8)/CH3CN = 18/82
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.8 µL

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LC

Samples
Theophylline , 1-Methylxanthine , 1,3-Dimethyluric acid
Applications
Analysis of Theophyline and metabolites
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
10mM CH3CO2NH4 (pH6.8) /CH3CN = 13/87
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
1.0 µL
Diluent
Dissolved samples in 0.1% NaOH aq and add acetonitrile into ratio to 90%

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LC

Samples
Allantoin , Xanthine , Hypoxanthine , Uric acid
Applications
Analysis of Uric acid metabolite
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
10mM CH3CO2NH4 (pH4.7) /CH3CN = 10/90
Frow rate
0.35 mL/min
Column temp.
30°C
Injection volume
1.0 µL
Diluent
Dissolved samples in 0.1% NaOH aq and add acetonitrile into ratio to 50%

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LC

Samples
Cyanuric acid , Ammelide , Melamine , Ammeline
Applications
Analysis of melamine compounds
Column
ULTRON AF-HILIC-CD
Column Size
100 mm L × 2.0 mm I.D.
Mobile phase
20mM HCO2NH4 (pH6.8) /CH3CN = 18/82
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
2.0 µL
Diluent
Dissolved samples in 0.1% NaOH aq and add acetonitrile into ratio to 50%

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